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| Annular Phase Plate Holes | Triple holes |
| Objective Magnification of Phase Plate | 10X 20X Phase Plate |
| Material | Plastic |
| Color | Black |
| Net Weight | 0.17kg (0.37lbs) |
| Applied Field | BM0303, FM0303, FM0304 Series Microscope |
Technical Info
| The conditions of different illumination of the microscope are a very important parameter. Choosing the correct illumination method can improve the resolution and contrast of the image, which is very important for observing the imaging of different objects. The wavelength of the light source is the most important factor affecting the resolution of the microscope. The wavelength of the light source must be smaller than the distance between the two points to be observed in order to be distinguished by the human eye. The resolution of the microscope is inversely proportional to the wavelength of the light source. Within the range of the visible light, the violet wavelength is the shortest, providing also the highest resolution. The wavelength of visible light is between 380~780nm, the maximum multiple of optical magnification is 1000-2000X, and the limit resolution of optical microscope is about 200nms. In order to be able to observe a much smaller object and increase the resolution of the microscope, it is necessary to use light having a much shorter wavelength as the light source. The most commonly used technical parameters for describing illumination are luminescence intensity and color temperature. Luminescence intensity, with lumen as unit, is the physical unit of luminous flux. The more lumens, the stronger the illumination. Color temperature, with K (Kelvin) as unit, is a unit of measure indicating the color component of the light. The color temperature of red is the lowest, then orange, yellow, white, and blue, all gradually increased, with the color temperature of blue being the highest. The light color of the incandescent lamp is warm white, its color temperature is 2700K, the color temperature of the halogen lamp is about 3000K, and the color temperature of the daylight fluorescent lamp is 6000K. A complex and complete lighting system can include a light source, a lampshade or lamp compartment, a condenser lens, a diaphragm, a variety of wavelength filters, a heat sink cooling system, a power supply, and a dimming device etc. Select and use different parts as needed. Of which, selection and use of the illuminating light source is the most important part of the microscope illumination system, as and other components are designed around the illuminating wavelength curve and characteristics of the illuminating light source. Some of the microscope light sources are pre-installed on the body or frame of the microscope, and some are independent. There are many types and shapes of light sources. Depending on the requirements of the microscope and the object to be observed, one type or multiple types of illumination at the same time can be selected. In addition, the whole beam and band adjustment of the light source, the position and illumination angle of the light source, and the intensity and brightness of the light all have a great influence on the imaging. For microscope imaging, a good lighting system may be a system that allows for more freedom of adjustment. In actual work, such as industry, too many adjustment mechanisms may affect the efficiency of use, therefore choose the appropriated configured lighting conditions is very important. |
| Phase contrast kit mainly includes annular diaphragm, phase contrast objective with a phase plate, and a phase telescope for central alignment. The annular diaphragm is placed near the aperture diaphragm, and there are single pieces, some are combined into a diaphragm group. There are also some that make the annular diaphragm into a turntable and combine together with the condenser. The annular diaphragm is to use a glass, coated with metal film, to block the light. When the light passes through the narrow slit of the diaphragm, it forms a hollow light cone, and different diaphragms are used corresponding to different objective lenses so as to generate diffraction and interference effects. The phase plate is mounted on the back image focal point of the objective lens. It is to use a piece of glass, coated with a light absorbing material (magnesium fluoride or other electrolyte) and a layer of metal film at the ring that is transparent to the corresponding annular diaphragm, so that the phase of direct or diffracted light passing through the phased annular diaphragm can be delayed by 1/4λ. The phase plate absorbs part of the light, extends the optical path and delays the phase of part of the light. After the two beams of light are combined, the interference is strengthened, and the vibration amplitude is increased or decreased, resulting in a phase difference, which makes various structural features more clear. Phase plates are usually used in two forms: Negative contrast: delay the direct light by 1/4λ. After the two sets of light waves are combined, the light waves are added, the vibration amplitude is increased, and the specimen structure becomes much brighter than the surrounding medium, forming a bright contrast. Positive contrast: delay the diffracted light by 1/4λ. After the two sets of light are combined, the light waves are subtracted, the vibration amplitude is reduced, and the specimen structure becomes much darker than the surrounding medium, forming a dark contrast. Annular diaphragm and phase plate adjustment Phase telescope works on the coaxial correction of the annular diaphragm and the phase plate. When using a phase-aligned telescope, it temporarily replaces one eyepiece, adjust and focus the image of the object on the phase plate, and then observe and adjust the auxiliary lens of the condenser. Adjust the annular diaphragm and the right corresponding to the phase plate to its concentric position, so that the beam of the annular diaphragm and the phase ring of the phase plate are the same size, and the annular beam is completely projected on the phase ring. Some microscopes have a Berrand lens that can be switched in the optical path, which is to correct the position of the annular diaphragm and phase plate. When using, screw the Bertrand lens into the optical path, adjust the focus and see the images of the annular diaphragm and the phase plate, after adjusting its position, rotate the Bertrand lens out of the optical path. |
| After unpacking, carefully inspect the various random accessories and parts in the package to avoid omissions. In order to save space and ensure safety of components, some components will be placed outside the inner packaging box, so be careful of their inspection. For special packaging, it is generally after opening the box, all packaging boxes, protective foam, plastic bags should be kept for a period of time. If there is a problem during the return period, you can return or exchange the original. After the return period (usually 10-30 days, according to the manufacturer’s Instruction of Terms of Service), these packaging boxes may be disposed of if there is no problem. |
| Packing | |
| Packaging Type | Carton Packaging |
| Packaging Material | Corrugated Carton |
| Inner Packing Material | Plastic Bag |
| Ancillary Packaging Materials | Sponge |
| Gross Weight | 0.27kg (0.60lbs) |
| Minimum Packaging Quantity | 1pc |
| Transportation Carton | Carton Packaging |
| Transportation Carton Material | Corrugated Carton |
| Transportation Carton Dimensions(1) | 15.2x15.2x15.2cm (6x6x6″) |
| Total Gross Weight of Transportation(kilogram) | 0.27 |
| Total Gross Weight of Transportation(pound) | 0.60 |
