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| Optical System | Infinite |
| Tube Lens Focal Length | 200mm |
| System Optical Magnification | 50-1000X |
| Trinocular Optical Magnification | 2.5-50X |
| Total Magnification | 50-1000X |
| Standard Eyepiece | 10X Adjustable Eyepiece |
| Standard Objective | 5X 10X 20X 50X 100X Infinity Plan Semi-Apochromatic Dark Field Metallurgical Objective |
| Standard Coupler | 0.5X |
| System Field of View | Dia. 0.25-5mm |
| System Working Distance | 1-20mm |
| Eye Tube Optical System | Infinite |
| Eye Tube Type | For Compound Microscope |
| Eye Tube Adjustment Mode | Siedentopf |
| Eye Tube Angle | 0-42° |
| Eye Tube Rotatable | 360° Degree Rotatable |
| Interpupillary Adjustment | 47-78mm |
| Eye Tube Inner Diameter | Dia. 30mm |
| Eye Tube Diopter Adjustable | Not Adjustable |
| Image Port Switch Mode | 0/100 Switch Trinocular |
| Eyepiece Type | Adjustable Eyepiece |
| Eyepiece Optical Magnification | 10X |
| Plan Eyepiece | Plan Eyepiece |
| Eyepiece Size for Eye Tube | Dia. 30mm |
| Eyepiece Field of View | Dia. 25mm |
| Eyepoint Type | High Eyepoint Eyepiece |
| Inward/Outward Nosepiece | Nosepiece Outward |
| Number of Holes on Nosepiece | Sextuple (6) Holes |
| Nosepiece Switch Mode | Motorized |
| Nosepiece with Slot | Slot with Analyzers / D.I.C. |
| Focus Mode | Manual |
| Coarse/Fine Focus Type | Coaxial Coarse/Fine Focus |
| Focus Distance | 32mm |
| Fine Focus Travel Distance | Same as Focus Distance |
| Fine Focus Minimum Scale | 1μm |
| XY Stage Travel Distance | 356x305mm |
| XY-Axis Drive Mode | Manual |
| Stage Platform Dimensions | 14 in. x12 in. |
| Illumination Type | HL Dual Illuminated Light |
| Top Illumination Type | Halogen Light |
| Bottom Illumination Type | LED |
| Coaxial Reflection Illuminator Light Source Type | Halogen Light |
| Coaxial Reflection Illuminator Input Voltage | DC 12V |
| Coaxial Reflection Illuminator Output Power | 100W |
| Condenser Max. Numerical Aperture | N.A. 0.65 |
| Coupler Magnification | 0.5X |
| Surface Treatment | Spray Paint |
| Material | Metal |
| Color | White |
| NX1000 | Nexcope-NX1000-Trinocular |
Technical Info
| A metallurgical microscope is a microscope that uses incident illumination (also known as reflection light) to observe the metallurgical structure of the surface of a metal specimen and perform microscopic analysis. Metallurgical microscopic analysis is widely involved in the material microstructure, internal components, state imaging, and qualitative and quantitative analysis, including the quantitative and spatial distribution of the phase and tissue structure, composition, crystallization and sub-crystallization, non-metallic inclusions, and tissue defects of the materials etc. Metallurgical microscope is one of the important components of industrial microscope. In addition to observing metal surface, metallurgical microscope plays an important role in metal heat treatment and cold processing. It is no longer limited to metal research, as it is also widely applied in other opaque or translucent objects, including fibers, soils, minerals, crystals, ceramics, surface treatments, integrated circuits, LCD screens, and other industries. Modern Metallurgical microscope not only have good optical systems, but also combine optical microscope, photoelectric conversion technology, and computer image processing technology to easily observe metallurgical images and analyze and rate metallurgical maps. The quality of the image is the primary indicator of metallurgical microscope. Metallurgical microscope needs the basic conditions of optical imaging such as high brightness, high contrast, high resolution and good color reproduction. At the same time, due to the environment applied, the microscope needs to be solid and durable. Metallurgical microscope generally uses coaxial reflection light method. The illuminating light passes through a coaxial reflection illuminator, after rotating a 90 degree angle, it is irradiated vertically (or nearly vertically) to the surface of the object to be observed, and then reflected back into the eyepiece through the objective lens. Notes on the Use of Metallurgical Microscope: When the observed metal surface is too rough, due to the diffusing effect of the incident light, the microscope cannot observe its internal structure, grinding and polishing of the surface of the metal sample must be carried out. However, during the process, no tissue structural change should occur on the surface; when some metal structures have a particularly strong surface reflection, it is necessary to use a certain reagent for corrosion treatment, dissolve some components, and thus see the morphology of the tissue. Metallurgical microscope are complex in function and diverse in components, and often large-scale metallurgical microscope are modular and require self-installation and commissioning. Be sure to read the operating manual first and carefully check the completeness of the components. Prior to installation and use, installation and adjustment of location of components are required. In particular, the positional adjustment of the light source in the coaxial reflection illuminator has a very big influence on the brightness and uniformity of the imaging illumination. For more information on the use of metallurgical microscopes, please refer to the Biological Microscope Biomicroscope on the BoliOptics website. |
| Microscopes and components have two types of optical path design structures. One type is finite optical structural design, in which light passing through the objective lens is directed at the intermediate image plane (located in the front focal plane of the eyepiece) and converges at that point. The finite structure is an integrated design, with a compact structure, and it is a kind of economical microscope. Another type is infinite optical structural design, in which the light between the tube lens after passing the objective lens becomes "parallel light". Within this distance, various kinds of optical components necessary such as beam splitters or optical filters call be added, and at the same time, this kind of design has better imaging results. As the design is modular, it is also called modular microscope. The modular structure facilitates the addition of different imaging and lighting accessories in the middle of the system as required. The main components of infinite and finite, especially objective lens, are usually not interchangeable for use, and even if they can be imaged, the image quality will also have some defects. The separative two-objective lens structure of the dual-light path of stereo microscope (SZ/FS microscope) is also known as Greenough. Parallel optical microscope uses a parallel structure (PZ microscope), which is different from the separative two-object lens structure, and because its objective lens is one and the same, it is therefore also known as the CMO common main objective. |
| The tube lens focal length is the focal length from the tube lens to the intermediate image plane of the design of infinite microscope, and its typical ranging is from 160 to 200 mm, depending on different manufacturers. |
| The magnification of the objective lens refers to the lateral magnification, it is the ratio of the image to the real size after the original image is magnified by the instrument. This multiple refers to the length or width of the magnified object. System optical magnification is the product of the eyepiece and the objective lens (objective lens zoom set) of the optical imaging part within the system. Optical magnification = eyepiece multiple X objective lens/objective lens set The maximum optical magnification of the microscope depends on the wavelength of the light to which the object is illuminated. The size of the object that can be observed must be greater than the wavelength of the light. Otherwise, the light cannot be reflected or transmitted, or recognized by the human eye. The shortest wavelength of ultraviolet light is 0.2 microns, so the resolution of the optical microscope in the visible range does not exceed 0.2 microns, or 200 nanometers. This size is converted to the magnification of the microscope, and it is the optical magnification of 2000X. Usually, the compound microscope can achieve 100X objective lens, the eyepiece is 20X, and the magnification can reach 2000X. If it is bigger, it will be called "invalid magnification", that is, the image is large, but the resolution is no longer increased, and no more details and information can be seen. |
| When the instrument is conducting electronic image magnification and observation through a camera or the like, the optically magnified portion may not be the optical path that passes through the "eyepiece-objective lens" of the instrument, at this time, the calculation method of the magnification is related to the third-party photo eyepiece passed. The trinocular optical magnification is equal to the multiplier product of objective lens (objective lens set) and the photo eyepiece Trinocular optical magnification = objective lens X photo eyepiece |
| Total magnification is the magnification of the observed object finally obtained by the instrument. This magnification is often the product of the optical magnification and the electronic magnification. When it is only optically magnified, the total magnification will be the optical magnification. Total magnification = optical magnification X electronic magnification Total magnification = (objective X photo eyepiece) X (display size / camera sensor target ) |
| Field of View, is also called FOV. The field of view, or FOV, refers to the size of the object plane (i.e., the plane of the point of the observed object perpendicular to the optical axis), or of its conjugate plane (i.e., object to primary image distance), represented by a line value. System field of view is the size of the actual diameter of the image of the terminal display device of the instrument, such as the size of the image in the eyepiece or in the display. Field of view number refers to the diameter of the field diaphragm of the objective lens, or the diameter of the image plane formed by the field diaphragm. Field of view number of objective lens = field of view number of eyepiece / (objective magnification / mechanical tube length) Large field of view makes it easy to observe the full view and more range of the observed object, but the field of view (FOV) is inversely proportional to the magnification and inversely proportional to the resolution, that is, the larger the field of view, the smaller the magnification, and also the lower the resolution of the object to be observed. There are usually two ways to increase the field of view, one is to replace with an objective lens of a smaller multiple, or to replace with an eyepiece of a smaller multiple. |
| Working distance, also referred to as WD, is usually the vertical distance from the foremost surface end of the objective lens of the microscope to the surface of the observed object. When the working distance or WD is large, the space between the objective lens and the object to be observed is also large, which can facilitate operation and the use of corresponding lighting conditions. In general, system working distance is the working distance of the objective lens. When some other equipment, such as a light source etc., is used below the objective lens, the working distance (i.e., space) will become smaller. Working distance or WD is related to the design of the working distance of the objective lens. Generally speaking, the bigger the magnification of the objective lens, the smaller the working distance. Conversely, the smaller the magnification of the objective lens, the greater the working distance. When it is necessary to change the working distance requirement, it can be realized by changing the magnification of the objective lens. |
| For siedentopf eyetube, when changing the interpupillary distance, it requires two hands pushing or pulling the two eyetubes left and right simultaneously, and the two eyepiece tubes or eyetubes will change their position at the same time. |
| Usually the Microscope Eyetube is 45°, some is 30°, Tiltable Eyetube Angle design of a microscope is also known as the ergonomics microscope. 0-30° or 0-45° is an ergonomic design. When the mechanical tube length / focal length of the tube of the microscope is relatively big, the microscope is relatively high, and the user's height or the seat of the work desk is not suitable, long-term use of microscope may cause sitting discomfort. Eyepiece tube with variable angle can freely adjust the angle without lowering the head. Especially when it is close to 0 degree and the human eye is close to horizontal viewing, long-time or long-term use can avoid fatigue damage to the cervical vertebra. |
| The eyepiece of the microscope can have different viewing or observing directions. When the position of the microscope is uncomfortable, the direction of the eyepiece tube of the microscope can be adjusted, to facilitate observation and operation. Placement method of different viewing angles of the microscope: General direction: the support column is behind the object to be observed Reverse direction: the support column is in front of the object to be observed Lateral direction: the support column is on the side of the object to be observed Rotating eyepiece tube, different microscopes may have different methods, for some, the direction is confirmed when installing the eyepiece tube of the microscope, for some, by rotating the body of the microscope, and for some, by rotating the support member on the support or holder of the microscope. |
| The distance between the two pupils of the human eye is different. When the image of exit pupil of the two eyepieces of the microscope are not aligned with the entry pupil of the eye, the two eyes will see different images, which can cause discomfort. Adjust the distance between the two eyepieces, to accommodate or adapt to the pupil distance of the observer's eyes. The adjustment range is generally between 55-75mm. |
| For most people, their two eyes, the left and the right, have different vision; for the eyepiece tube, the eyepoint height of the eyepiece can be adjusted to compensate for the difference in vision between the two eyes, so that the imaging in the two eyes is clear and consistent. The range of adjustment of the eyepiece tube is generally diopter plus or minus 5 degrees, and the maximum differential value between the two eyepieces can reach 10 degrees. Monocular adjustable and binocular adjustable: some microscopes have one eyepiece tube adjustable, and some have two eyepiece tubes adjustable. First, adjust one eyepiece tube to the 0 degree position, adjust the microscope focusing knob, and find the clear image of this eyepiece (when the monocular adjustable is used, first adjust the focusing knob to make this eyepiece image clear), then adjust the image of another eyepiece tube (do not adjust the focusing knob again at this time), repeatedly adjust to find the clear position, then the two images are clear at the same time. For this particular user, do not adjust this device anymore in the future. As some microscopes do not have the vision adjustment mechanism for the eyepiece tube, the vision of the two eyes are adjusted through the eyepiece adjustable. |
| The third eyepiece splitting in the trinocular microscope is to borrow one of the two sets of eyepiece optical paths as the photographic light path. The beam split prism or beam splitter can reflect part of the image light to the eyepiece, and part passes through to the third eyepiece photographic light path, such a trinocular microscope is called trinocular simultaneous imaging microscope, or true-trinocular. The beam split prism or beam splitter of the trinocular simultaneous imaging microscope or true-trinocular often has different splitting modes, such as 20/80 and 50/50, etc. Usually, the former is the luminous flux ratio of the eyepiece optical path, and the latter is the luminous flux ratio of the photographic optical path. The advantage of true-trinocular is that, the real three optical paths can be imaged at the same time, and are not affected by the simultaneous use of the eyepiece observation and the photographic optical path (display). The disadvantage is that, because of the reason of the splitting, the image light of the photography is only a part. In theory, the image effect will be affected, and the effect is more obvious in the binocular eyepiece observation. If viewed closely, one will find that the eyepiece of the light path is relatively dark. However, in the current optical design and materials, the impact on the actual work is not very big, especially in the observation of low magnification objective lens, it has basically no effect at all, and therefore used by many people. |
| The adjustable eyepiece is between the lens of the eyepiece and the focal plane, with distance adjustable device. For most people, their two eyes, the left and the right, have different vision. For adjustable eyepieces, the eyepoint height of the eyepiece can be adjusted to compensate for the difference in vision between the two eyes, making the image in the two eyes clear and consistent. The range of adjustment of the general eyepiece is that the diopter is plus or minus 5 degrees, and the maximum difference between the two eyepieces can reach 10 degrees. Before use, it is generally necessary to adjust both eyepieces to the initial position where the scale is displayed as 0, which is used as a baseline to facilitate up and down adjustment. The reticle position of the eyepiece is generally 10mm below the fixed position of the eyepiece tube. Because the vision of each person is different, some people may not be able to see the reticle clearly. For adjustable eyepiece, the height of the reticle position can be adjusted to make the reticle and the observed object clear at the same time, this is the advantage of adjustable eyepiece that mounts the diopter adjustment on the eyepiece tube compared with non-adjustable eyepiece. When non-adjustable eyepiece is equipped with a reticle, if the diopter is adjusted, the reticle will rotate accordingly, thereby affecting the position of the measurement. For adjustable eyepiece, when its diopter is adjusted, its reticle does not rotate. |
| Eyepiece optical magnification is the visual magnification of the virtual image after initial imaging through the eyepiece. When the human eye observes through the eyepiece, the ratio of the tangent of the angle of view of the image and the tangent of the angle of view of the human eye when viewing or observing the object directly at the reference viewing distance is usually calculated according to 250 mm/focal length of eyepiece. The standard configuration of a general microscope is a 10X eyepiece. Usually, the magnification of the eyepiece of compound microscope is 5X, 8X, 10X, 12.5X, 16X, 20X. As stereo microscope has a low total magnification, its eyepiece magnification generally does not use 5X, but can achieve 25X, 30X and other much bigger magnification. |
| The eyepiece field of view is the diameter of the field diaphragm of the eyepiece, or the diameter of the image plane of the field diaphragm imaged by the field diaphragm. The diameter of a large field of view can increase the viewing range, and see more detail in the field of view. However, if the field of view is too large, the spherical aberration and distortion around the eyepiece will increase, and the stray light around the field of view will affect the imaging effect. |
| Eye point refers to the axial distance between the upper end of the metal frame of the eyepiece and the exit of pupil. The exit of pupil distance of high eyepoint eyepiece is farther than that of the eye lens of the ordinary eyepiece. When this distance is greater than or equal to 18mm, it is a high eyepoint eyepiece. When observing, one does not need to be too close to the eyepiece lens, making it comfort to observe, and it can also be viewed with glasses. Generally, there is a glasses logo on the eyepiece, indicating that it is a high eyepoint eyepiece. |
| The nosepiece of a microscope is generally switched manually. A motarized nosepiece is to add an electric motor onto the nosepiece to control switching of the nosepiece through the electric switch, so as to switch the objective used. This device can be added when some microscopes are bulky, switching of the objective needs to be kept steady, and needs to be frequently switched. |
| The nosepiece has a slot for mounting polarizers, filters and other devices. |
| Focus mechanism, the coarse / fine focus knobs are in a coaxial center position, they are connected together by a gear reduction mechanism, which can be coarse/ fine focus adjusted at any time during the entire stroke. Generally, the coarse focus diameter is relatively big, which is inside close to the body of the microscope, and the fine focus diameter is relatively small, which is outside of the body of the microscope. Coarse focus adjustment is used to quickly move to find the image, and the fine focus adjustment is used to finely adjust the clarity of the image. Generally, the minimum read value of the fine focus adjustment can be accurate to 1 micron, and single circle can reach a stroke of 0.1 mm. Mechanical fine focus plays a very important role in the accuracy of the microscope resolution. If the fine focus accuracy is not enough, or cannot be stabilized at the sharpest focusing position, the image will be out of focus and become blurred. The tightness of coarse focus is generally adjustable. Generally, on one side of the knob (usually on the right side), there is a textured knob on the inside of the coarse knob, which is tightened if rotated clockwise; and loosened if rotated counterclockwise. In the process of focusing, direct focusing should not be on the objective of high magnification; instead, find the object of low magnification first, and gradually adjust to high magnification. Usually, the coarse focus knob is rotated first, and when the objective lens is gradually lowered or the platform is gradually rising, find the object, and then adjust with the fine focus, until the object image in the field of view is clear. Generally, when changing from low magnification to high magnification objective, one only need to slightly adjust the fine focus knob to make the object image clear. During the process, the distance between the objective and the specimen should be observed from the side, to understand the critical value of the object distance between the lens and the specimen. When using a high magnification objective, since the distance between the objective and the specimen is very close, after the image is found, the coarse focus knob cannot generally be used, and the fine focus knob can only be used to avoid excessive distance of movement, damaging the objective and the slide or specimen. By using the characteristics of the fine focus, the height or thickness of the observed object can be roughly measured under the microscope, such as measuring the thickness of the cell or tissue, the thickness of the cover glass, and the thickness of small objects that cannot be measured by various conventional measuring instruments. Method of measurement: place the object to be measured at the center of the field of view of the stage. After the image is clearly focused, try to use the highest magnification objective as much as possible, and align the adapter of the top feature point of the object to be measured. After adjusting clear, record the position of scale of the fine focus knob. Then, move the objective down to the adapter of the lowest feature point of the object to be measured, and record the position of scale of the fine focus knob. Then, according to the above fine focus, record the number of rounds of movement, and based on the parameters of conversion of each round into stroke (see the microscope fine focus knob parameters), the number of rounds is converted into the total stroke, which is the height of the object to be measured. If it is repeated a few times for average, a more accurate measurement can be obtained. |
| Coupler/C-mount adapter is an adapter commonly used for connection between the C-adapter camera (industrial camera) and a microscope. |
| Coupler magnification refers to the line field magnification of the coupler/C-mount-adapter. With different magnifications of the adapter lens, images of different magnifications and fields of view can be obtained. The size of the image field of view is related to the sensor size and the coupler/C-mount-adapter magnification. Camera image field of view (mm) = sensor diagonal / coupler/C-mount-adapter magnification. For example: 1/2 inch sensor size, 0.5X coupler/C-mount-adapter coupler, field of view FOV (mm) = 8mm / 0.5 = 16mm. The field of view number of the microscope 10X eyepiece is usually designed to be 18, 20, 22, 23mm, less than 1 inch (25.4mm). Since most commonly used camera sensor sizes are 1/3 and 1/2 inches, this makes the image field of view on the display always smaller than the field of view of the eyepiece for observation, and the visual perception becomes inconsistent when simultaneously viewed on both the eyepiece and the display. If it is changed to a 0.5X coupler/C-mount-adapter, the microscope image magnification is reduced by 1/2 and the field of view is doubled, then the image captured by the camera will be close to the range observed in the eyepiece. Some adapters are designed without a lens, and their optical magnification is considered 1X. |
